The effectiveness of academic environments depends on the harmonious interaction between academic processes and the indoor environment. However, the absence of thermal comfort can disrupt this complex dynamic. Prolonged exposure to adverse thermal conditions can lead to reduced productivity and overall satisfaction with the learning environment. Many students' classes are held after the noon prayer, and the type of interaction people have with water in Islamic culture is through ablution and washing hands and face, which adjusts the temperature of the body's skin surface and, as a result, improves the thermal comfort of individuals. The present study aims to investigate the extent of thermal comfort of students after ablution compared to students without ablution at time levels of 30, 60, 90,120 minutes in academic environments. This study was tested by simulating the academic environment in a climatic chamber with preset temperatures in the range of (17, 21, 25, 29) degrees Celsius. Participants, including 20 men, were randomly divided into 4 groups in two experimental conditions: one day with ablution and the next day without ablution at time levels of 30, 60, 90, 120 minutes. The method used in this study was a laboratory study and was performed using within-case statistical analysis with SPSS27 software. The results showed that performing ablution before entering the climatic chamber can improve the thermal comfort range of individuals by 1.5 hours more than those who do not perform ablution.