en گروه بایومواد Biomaterials Research Paper Research Paper <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><span style="font-size:14.0pt"><span style="background:yellow"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="background-color:#ffffff;">In the current study, titanium dioxide (TiO₂)</span></span></span></span></span><span style="font-size:14.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="background-color:#ffffff;"> nanoparticles were synthesized and subsequently combined with chitosan (CS) and silver (Ag) to augment their antimicrobial effectiveness. The synthesized TiO₂, TiO₂-CS, and TiO₂-CS-Ag nanocomposites were subjected to </span><span style="background:yellow"><span style="background-color:#ffffff;">various characterization analyses in order </span></span><span style="background-color:#ffffff;">to thoroughly assess their structural, morphological, and compositional attributes. XRD analysis substantiated the phase transition from anatase to rutile consequent </span><span style="background:yellow"><span style="background-color:#ffffff;">to </span></span><span style="background-color:#ffffff;">incorporation of chitosan and silver, accompanied by a diminution in nanoparticle dimensions. FTIR spectra corroborated the existence of functional groups linked to chitosan and silver, while FESEM illustrated morphological modifications, notably the emergence of polygonal nanostructures within the TiO₂-CS-Ag composite. The antibacterial efficacy of the synthesized nanocomposites was evaluated against </span><i><span style="background-color:#ffffff;">Escherichia coli</span></i><span style="background-color:#ffffff;"> (</span><i><span style="background-color:#ffffff;">E. coli</span></i><span style="background-color:#ffffff;">) and </span><i><span style="background-color:#ffffff;">Staphylococcus aureus</span></i><span style="background-color:#ffffff;"> (</span><i><span style="background-color:#ffffff;">S. aureus</span></i><span style="background-color:#ffffff;">)</span><span style="background:yellow"><span style="background-color:#ffffff;">.</span></span><span style="background-color:#ffffff;"> Although pure TiO₂ demonstrated minimal antibacterial properties, the TiO₂-CS and TiO₂-CS-Ag composites exhibited substantial inhibition zones, with the most pronounced efficacy recorded for the TiO₂-CS-Ag composite attributable to the synergistic interaction between chitosan and silver nanoparticles. BET analysis revealed that the augmented antimicrobial activity was associated with the increased surface area of the TiO₂-CS-Ag nanocomposite. </span></span></span></span></span></span></span><br> <span style="background-color:#ffffff;">&nbsp;</span> TiO₂ nanocomposite,chitosan,silver nanoparticles,antibacterial activity,green synthesis 55 63 http://ijmse.iust.ac.ir/browse.php?a_code=A-10-5999-1&slc_lang=en&sid=1 Muayad Raheem Hussein abs951@yahoo.com 1800319475328460022033 1800319475328460022033 No University of sumer, college of science, physics department, Thi-Qar, Iraq. Sabah Hasan jumaah boshrags@yahoo.com 1800319475328460022034 1800319475328460022034 Yes Mustansiriyah university, college of science, physics department, Baghdad, Iraq. Ali N. Majees ali.n.majeed@src.edu.iq 1800319475328460022035 1800319475328460022035 No Scientific research commission, Baghdad, Iraq