en Research Paper Research Paper <strong>Abstract</strong><br> In this research, Gelatine (GEL)/ Chitosan (CH) wound dressing was prepared and irradiated with gamma rays from ⁶⁰Co source for wound healing applications. GEL-CH composite characterization and functional properties were determined. The structural changes occurring after &gamma;-irradiation at doses from 5 to 25 kGy were reported by physico-chemical techniques such as Electron Paramagnetic Resonance (EPR), Fourier Transform Infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Electrochemical Impedance Spectroscopy (EIS) studies. The antioxidant capacity was studied using DPPH (1,1-diphenyl-2-picrylhydrazyl free radical) scavenging and the antibacterial activities of <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> were observed using liquid medium. Results revealed that EPR spectroscopy of un-irradiated GEL-CH showed 2 paramagnetic centers correspond to <em>g=</em>2.077 and<em> g=</em> 2.079. After irradiation, no active centre was appeared. A dose-dependent decrease in the central signal intensity was detected, then the EPR signal intensity almost disappears at 20 kGy. Gamma rays caused a slight increase in ion conductivity. FTIR suggest a slightly crosslinking phenomenon at 20 kGy. The XRD analysis does not show peak indicating crystallinity between a range of 2&theta; (15&ndash;30&deg;). Moreover, &gamma;-irradiation&nbsp;elevated the Scavenging DPPH radical activity (0.75 &plusmn; 0.07%). Gamma rays did not affect the antibacterial activity of GEL-CH wound dressing against pathogenic bacteria. The innovative results showed that the required &gamma;-radiation for sterilization was ranged from 5 to 25 kGy. It permits to improve the physico-chemical and biological properties and maintain the native structural integrity of the GEL/ COL wound dressing Keywords: Gelatin,Chitosan,Wound Dressing,γ-irradiation,Electron Paramagnetic Resonance 1 8 http://ijmse.iust.ac.ir/browse.php?a_code=A-10-4259-1&slc_lang=en&sid=1 Salma Bessalah 1800319475328460014848 1800319475328460014848 No 1Livestock and Wildlife Laboratory, Arid Lands Institute (I.R.A), University of Gabès, Médenine,Tunisia Samira Jbahi jbahisamira@yahoo.fr 1800319475328460014849 1800319475328460014849 Yes 2Energy and Matter Research Laboratory, Centre National des Sciences et 3TechnologiesNucléaires (CNSTN), Pole technologique, 2020, Sidi Thabet-Tunisia Mouldi Zagrouba 1800319475328460014850 1800319475328460014850 No 3laboratory of physics materials, faculty of sciences, University of Sfax, 3000 B.P1171,Tunisia Hajji Sawsen 1800319475328460014851 1800319475328460014851 No 2Energy and Matter Research Laboratory, Centre National des Sciences et 3TechnologiesNucléaires (CNSTN), Pole technologique, 2020, Sidi Thabet-Tunisia Amel Raoufi 1800319475328460014852 1800319475328460014852 No 2Energy and Matter Research Laboratory, Centre National des Sciences et 3TechnologiesNucléaires (CNSTN), Pole technologique, 2020, Sidi Thabet-Tunisia Mustpha Hidouri 1800319475328460014853 1800319475328460014853 No 4High Institute of Applied Sciences and Technology, Gabes University, Omar Ibn Khattab Road, 6029, Tunisia